Sustained Elevation of Intracellular Cyclic 3’-S’ Adenosine Monophosphate Is Necessary for Preservation of Platelet Integrity During Long-Term Storage at 22°C
نویسندگان
چکیده
Preservation of platelet integrity and responsiveness was examined in platelet concentrates prepared in the presence of various formulations and combinations of plateletactivation inhibitors affecting intracellular levels of cyclic 3-5’ adenosine monophosphate (CAMP). Platelet concentrates were prepared and stored in an artificial medium for two weeks at 22’C. Markers of metabolic activity (pH, lactate, p02, pCOz in the medium), aggregation response, hypotonic shock response, and glycoprotein Ib (GPIb) expression were assessed along with direct measurements of cAMP in platelet pellets and thromboxane Bz (TxB,) in the supernate. The platelet concentrates prepared with only adenylate-cyclase stimulators (prostaglandin El or forskolin) showed less maintenance of the integrity and re-
منابع مشابه
Sustained elevation of intracellular cyclic 3'-5' adenosine monophosphate is necessary for preservation of platelet integrity during long-term storage at 22 degrees C.
Preservation of platelet integrity and responsiveness was examined in platelet concentrates prepared in the presence of various formulations and combinations of platelet-activation inhibitors affecting intracellular levels of cyclic 3'-5' adenosine monophosphate (cAMP). Platelet concentrates were prepared and stored in an artificial medium for two weeks at 22 degrees C. Markers of metabolic act...
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